normal human dermal fibroblasts hdfs  (PromoCell)

Journal: International Journal of Molecular Sciences

Article Title: A Simple Ionic-Gelation Method for Chitosan Nanoparticle Synthesis and Standardized Protocols for Biological Safety Assessment: Antibacterial Activity, Phytotoxicity, and Biocompatibility

doi: 10.3390/ijms27083673

Figure Lengend Snippet: Cell viability of HDF cells treated with Ch NPs at concentrations of 2.5–160 µg/mL for 24 h. Viability was determined using the MTT assay and expressed as mean ± SE (n = 3). Different letters indicate statistically significant differences ( p < 0.05).

Article Snippet: The biocompatibility of Ch NPs was evaluated using primary HDF (ATCC ® PCS-201-012TM [ ], American Type Culture Collection, Manassas, VA, USA) and HaCaT cells (Cat. No. T0020001, AddexBio, San Diego, CA, USA).

Techniques: MTT Assay

Journal: Biomedicines

Article Title: Mesenchymal Stem Cell–Derived Exosomes Mitigate Cutaneous Radiation Injury Through Coordinated Modulation of DNA Repair, Stress, and Inflammatory Gene Programs

doi: 10.3390/biomedicines14040811

Figure Lengend Snippet: Expression of early damage and stress-response genes ( a ) FDXR , ( b ) GADD45A , ( c ) SESN1 , and ( d ) GDF15 in irradiated HDFs (10 Gy) treated with exosomes (1 × 10 9 particles/mL) derived from BM-MSCs (BM-IR) and UC-MSCs (UC-IR), measured by qPCR. Gene expression was normalized to GAPDH and expressed as fold change relative to the non-irradiated Vehicle control at each timepoint. Data are shown as mean ± SD of three technical replicates from a representative experiment performed using a single HDF donor. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by ordinary one-way ANOVA followed by Dunnett’s post hoc test, comparing each treatment group to the Vehicle-IR control at the corresponding timepoint.

Article Snippet: Primary human dermal fibroblasts (HDFs) were purchased from PromoCell GmbH (SKU: C-12302; Heidelberg, Germany).

Techniques: Expressing, Irradiation, Derivative Assay, Gene Expression, Control

Journal: Biomedicines

Article Title: Mesenchymal Stem Cell–Derived Exosomes Mitigate Cutaneous Radiation Injury Through Coordinated Modulation of DNA Repair, Stress, and Inflammatory Gene Programs

doi: 10.3390/biomedicines14040811

Figure Lengend Snippet: Expression of DNA repair and antioxidant genes ( a ) DDB2 , ( b ) RNF8 , and ( c ) SOD1 in irradiated HDFs (10 Gy) treated with exosomes (1 × 10 9 particles/mL) derived from BM-MSCs (BM-IR) and UC-MSCs (UC-IR), measured by qPCR. Gene expression was normalized to GAPDH and expressed as fold change relative to the non-irradiated Vehicle control at each timepoint. Data are shown as mean ± SD of three technical replicates from a representative experiment performed using a single HDF donor. * p < 0.05, ** p < 0.01, *** p < 0.001, by ordinary one-way ANOVA followed by Dunnett’s post hoc test comparing each treatment group to the Vehicle-IR control at the corresponding timepoint.

Article Snippet: Primary human dermal fibroblasts (HDFs) were purchased from PromoCell GmbH (SKU: C-12302; Heidelberg, Germany).

Techniques: Expressing, Irradiation, Derivative Assay, Gene Expression, Control

Journal: Biomedicines

Article Title: Mesenchymal Stem Cell–Derived Exosomes Mitigate Cutaneous Radiation Injury Through Coordinated Modulation of DNA Repair, Stress, and Inflammatory Gene Programs

doi: 10.3390/biomedicines14040811

Figure Lengend Snippet: Expression of cell-cycle regulation and proliferation genes ( a ) CDKN1A , ( b ) CDKN2A , ( c ) MKI67 , ( d ) H2AFX , and ( e ) VEGFA in irradiated HDFs (10 Gy) treated with exosomes (1 × 10 9 particles/mL) derived from BM-MSCs (BM-IR) and UC-MSCs (UC-IR), measured by qPCR. Gene expression was normalized to GAPDH and expressed as fold change relative to the non-irradiated Vehicle control at each timepoint. Data are shown as mean ± SD of three technical replicates from a representative experiment performed using a single HDF donor. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by ordinary one-way ANOVA followed by Dunnett’s post hoc test, comparing each treatment group to the Vehicle-IR control at the corresponding timepoint.

Article Snippet: Primary human dermal fibroblasts (HDFs) were purchased from PromoCell GmbH (SKU: C-12302; Heidelberg, Germany).

Techniques: Expressing, Irradiation, Derivative Assay, Gene Expression, Control

Journal: Biomedicines

Article Title: Mesenchymal Stem Cell–Derived Exosomes Mitigate Cutaneous Radiation Injury Through Coordinated Modulation of DNA Repair, Stress, and Inflammatory Gene Programs

doi: 10.3390/biomedicines14040811

Figure Lengend Snippet: Expression of inflammatory response genes ( a ) IL-6 and ( b ) TNFAIP3 in irradiated HDFs (10 Gy) treated with exosomes (1 × 10 9 particles/mL) derived from BM-MSCs (BM-IR) and UC-MSCs (UC-IR), measured by qPCR. Gene expression was normalized to GAPDH and expressed as fold change relative to the non-irradiated Vehicle control at each timepoint. Data are shown as mean ± SD of three technical replicates from a representative experiment performed using a single HDF donor. * p < 0.05, ** p < 0.01, **** p < 0.0001 by ordinary one-way ANOVA followed by Dunnett’s post hoc test, comparing each treatment group to the Vehicle-IR control at the corresponding timepoint.

Article Snippet: Primary human dermal fibroblasts (HDFs) were purchased from PromoCell GmbH (SKU: C-12302; Heidelberg, Germany).

Techniques: Expressing, Irradiation, Derivative Assay, Gene Expression, Control